這個禮拜三Group meeting的報告是負責介紹Staining
包含Stain history, Wright’s stain, Giemsa stain, Liu’s stain, PAS stain (bonus)
上個禮拜做了很多片的Liu’s stain & PAS stain
查了資料之後,這下對Staining的了解又更多了:)
Liu’s stain
Procedure
- Cover the slide with 5~6 drops of Liu A. Wait 30 seconds.
- Add 2 times of Liu B on the slide. Mix well by blowing the surface. Wait 60 seconds.
- Wash off the staining solution with running tap water(at the back side).
- Dry and cover
PAS stain
- Procedure
- Warm the Schiff’s reagent to room temperature
- De-wax and hydrate paraffin sections
- Oxidize for 10-30 min in periodic acid
- Wash in running tap water for 3 min
- Immerse in Schiff’s reagent for 20 min
- Wash in running tap water for 10 min
- Apply counterstains, as desired ( hematoxylin, 90 sec)
- Dehydration in alcohol
- Clear in xylene and cover
完整報告(pdf)
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